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Detection Image Detection method details

Name:

 QL-TAX-PS-001

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the pea lectin gene (EU-RL GMFF as of January 23, 2017)

Comment:

 Target is the pea (Pisum sativum) lectin (psl) gene

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 taxon-specific

Target Species name:

 Pisum sativum

  • Oligonucleotides:

  • Forward Primer

  • Name:

    lecPea1-F

  • Sequence:

    TGGAATCGATGTGAACAGTATCAA

  • Size:

    24

  • Reverse Primer

  • Name:

    lecPea1-R

  • Sequence:

    ACAACATTAGCCTCTTCACCATTCT

  • Size:

    25

  • Probe

  • Name:

    lecPea1-P

  • Sequence:

    TCCGTAAACACTAAGTCGTGGAAGTTG

  • Size:

    27

  • Amplicon:

  • Sequence:

    tggaatcgatgtgaacagtatcaaatccgtaaacactaagtcgtggaagttgcagaatggtgaagaggctaatgttgt

  • Size:

    78

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-TAX-PS-001Link to the document url. EU reference method protocol