Detection method details
Name: |
QL-ELE-00-023 |
Description: |
Qualitative real-time PCR (SYBRGreen) method for detection of T-35S pCAMBIA sequences (EU-RL GMFF GMOMETHODS database as of January 20, 2017). |
Comment: |
Target is the Cauliflower mosaic virus 35S terminator (CaMV T-35S) in pCAMBIA vector. |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
T-Cauliflower mosaic virus
|
-
Oligonucleotides:
Forward Primer
Name:
t35S pCAMBIA c-FSequence:
CGGGGGATCTGGATTTTAGTASize:
21
Reverse Primer
Name:
t35S pCAMBIA a-RSequence:
AGGGTTCCTATAGGGTTTCGCTCSize:
23
-
Amplicon:
Sequence:
cgggggatctggattttagtactggattttggttttaggaattagaaattttattgatagaagtattttacaaatacaaatacatactaagggtttcttatatgctcaacacatgagcgaaaccctataggaaccctSize:
137
Documents
| Citation | Type | Local copy |
|---|---|---|