Detection method details
Name: |
QT-EVE-DBN9501-qF/DBN9501-qR |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event DBN9501 (Wang et al., 2023) |
Comment: |
Target is the 3' integration border region (IBR) between the insert of maize event DBN9501 and the maize host genome (included 65 bp T-DNA and 31 bp maize genome DNA sequence). |
Validation: |
ring trial validation |
Standardisation: |
unknown |
Related Methods: |
QT-TAX-zSSIIb-3F/zSSIIb-4R |
Type: |
event-specific |
Target GMO name: |
DBN9501 |
-
Oligonucleotides:
Forward Primer
Name:
DBN9501-qFSequence:
AACGTGACTCCCTTAATTCTCCSize:
22
Reverse Primer
Name:
DBN9501-qRSequence:
CCGGACTACTATACCATTTATAGTTACASize:
28
Probe
Name:
DBN9501-qPSequence:
ACTGAAGGCGGGAAACGACAATCTSize:
24
-
Amplicon:
Sequence:
aacgtgactcccttaattctccgctcatgatcagattgtcgtttcccgccttcagtttaaactatcagtgtaactataaatggtatagtagtccggSize:
96