Detection method details
Name: |
QT-ELE-00-002 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of phosphinothricin N-acetyltransferase gene (Weighardt et al., 2004) (EURL GMFF GMOMETHODS database as of February 5, 2020) |
Comment: |
Target is the phosphinothricin N-acetyltransferase (pat) gene from Streptomyces viridochromogenes |
Validation: |
ring trial validation |
Standardisation: |
unknown |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-pat-STRVR
|
-
Oligonucleotides:
Forward Primer
Name:
KVM-5Sequence:
TTGAGGGTGTTGTGGCTGGTASize:
21
Reverse Primer
Name:
KVM-6Sequence:
TGTCCAATCGTAAGCGTTCCTSize:
21
Probe
Name:
Pat1Sequence:
CTTCCAGGGCCCAGCGTAAGCASize:
22
-
Amplicon:
Sequence:
ttgagggtgttgtggctggtantgcttacgctgggccctggaagnnnaggaacgcttacgattggacaSize:
68
Documents
Citation | Type | Local copy |
---|---|---|
Weighardt F., Barbati C., Paoletti C., Querci M., Kay S., De Beuckeleer M., Van den Eede G.; Real-time polymerase chain reaction-based approach for quantification of the pat gene in the T25 Zea mays event J AOAC Int 87:1342-1355 (2004) | peer-reviewed article | |
EU-RL GMFF GMOMETHODS database; QT-ELE-00-002 | EU reference method protocol |