Detection method details
Name: |
QL-ELE-00-018 |
Description: |
Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014) |
Comment: |
Target is the T-nos-RHIRD from Agrobacterium tumefaciens |
Validation: |
ring trial validation |
Standardisation: |
unknown |
Related Methods: |
QL-TAX-BN-003, QL-TAX-GM-001, QL-TAX-ZM-002 |
Type: |
element-specific |
Target DNA element: |
T-nos-RHIRD
|
-
Oligonucleotides:
Forward Primer
Name:
tNOS_NN_FwdSequence:
GATTAGAGTCCCGCAATTATACATTTAASize:
28
Reverse Primer
Name:
tNOS D REVSequence:
TTATCCTAGKTTGCGCGCTATATTTSize:
25
-
Amplicon:
Sequence:
gattagagtcccgcaattatacatttaannnnnnnnnnnnnnnnaaatatagcgcgcaamctaggataaSize:
69
Documents
| Citation | Type | Local copy |
|---|---|---|