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Detection Image Detection method details

Name:

 QL-ELE-00-016

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6).

Comment:

 Targets are synthetic cry1Ab, cry1Ac or cry1Ab/Ac fusion genes derived from Bacillus thuringiensis.

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 element-specific

Target DNA element:
CS-cry1Ab_1Ac_tr-SYNTH, CS-cry1Ac-BACTU, CS-cry1Ab-BACTU

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Bt-F1(mod)

  • Sequence:

    GAGGAAATGCGTATTCAATTCAAC

  • Size:

    24

  • Reverse Primer

  • Name:

    Bt-R

  • Sequence:

    TTCTGGACTGCGAACAATGG

  • Size:

    20

  • Probe

  • Name:

    Bt-P

  • Sequence:

    ACATGAACAGCGCCTTGACCACAGC

  • Size:

    25

  • Amplicon:

  • Sequence:

    gaggaaatgcgtattcaattcaacgacatgaacagcgccttgaccacagctatcccattgttcgcagtccagaa

  • Size:

    74

Documents

Citation Type Local copy
Xu, W., Cao, S., He, X., Luo, Y., Guo, X., Yuan, Y., Huang, K. (2009). Safety assessment of Cry1Ab/Ac fusion protein. Food and Chemical Toxicology, 47(7), 1459-1465.Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QL-ELE-00-016Link to the document url. EU reference method protocol
Grisolia, C. K., Oliveira, R., Domingues, I., Oliveira-Filho, E. C., Monerat, R. G., & Soares, A. M. V. M. (2009). Genotoxic evaluation of different delta-endotoxins from Bacillus thuringiensis on zebrafish adults and development in early life stages. Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 672(2), 119-123.Link to the document url. peer-reviewed article