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Detection Image Detection method details

Name:

 QL-CON-00-002

Description:

 Qualitative conventional PCR method for detection of tomato event Nema282F (ISO/FDIS 21569)

Comment:

 Target is the junction region between the polygalacturonase (PG) gene copy from Solanum lycopersicum L. and the nopaline synthase terminator (T-nos) from Agrobacterium tumefaciens of the introduced construct. The amplicon identity is confirmed by Southern blotting with a labelled specific DNA probe or by restriction digestion.

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

 QL-TAX-SL-001

Type:

 construct-specific

Target GMO names:
Nema282F

  • Oligonucleotides:

  • Forward Primer

  • Name:

    PG34L

  • Sequence:

    GGATCCTTAGAAGCATCTAGT

  • Size:

    21

  • Reverse Primer

  • Name:

    t-Nos

  • Sequence:

    CATCGCAAGACCGGCAACAG

  • Size:

    20

  • Amplicon:

  • Sequence:

    ggatccttagaagcatctagtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnctgttgccggtcttgcgatg

  • Size:

    350

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-CON-00-002Link to the document url. EU reference method protocol