Help icon

Detection Image Detection method details

Name:

 QT-TAX-OS-003

Description:

 Quantitative real-time PCR (TaqMan) method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009)

Comment:

 Target is the endogenous sucrose-phosphate synthase (SPS) gene of Oryza sativa

Validation:

 ring trial validation

Standardisation:

 unknown

Related Methods:

Type:

 taxon-specific

Target Species name:

 Oryza sativa

  • Oligonucleotides:

  • Forward Primer

  • Name:

    SPS-1F

  • Sequence:

    TTGCGCCTGAACGGATAT

  • Size:

    18

  • Reverse Primer

  • Name:

    SPS-3R

  • Sequence:

    CGGTTGATCTTTTCGGGATG

  • Size:

    20

  • Probe

  • Name:

    SPS-P

  • Sequence:

    TCCGAGCCGTCCGTGCGTC

  • Size:

    19

  • Amplicon:

  • Sequence:

    ttgcgcctgaacggatatnnnnnnnnnnnnnnnnnnnnnnngacgcacggacggctcggancatcccgaaaagatcaaccg

  • Size:

    81

Documents

Citation Type Local copy
Jiang L., Yang L., Zhang H., Guo J., Mazzara M., Van den Eede G., Zhang D.; International collaborative study of the endogenous reference gene, sucrose phosphate synthase (SPS), used for qualitative and quantitative analysis of genetically modified riceLink to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QT-TAX-OS-003Link to the document url. EU reference method protocol