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Detection Image Detection method details

Name:

 QL-EVE-ZM-P1waxy/P2waxy

Description:

 Qualitative real-time PCR for the detection of maize event GE-CRISPR-Cas Waxy Corn (Gao et al., 2020).

Comment:

 Target is the 4 kb deletion junction in the maize waxy gene locus.

Validation:

 unknown

Standardisation:

 none

Related Methods:

Type:

 event-specific

Target GMO name:

 GE-CRISPR-Cas Waxy Corn

  • Oligonucleotides:

  • Forward Primer

  • Name:

    P1waxy

  • Sequence:

    GTGTGCGTGCGTGCAGAC

  • Size:

    18

  • Reverse Primer

  • Name:

    P2waxy

  • Sequence:

    AGCAGGGATTATTTACTCCACCAC

  • Size:

    24

  • Probe

  • Name:

    Probe1waxy

  • Sequence:

    CAAGCCAAGGCGAGG

  • Size:

    15

  • Amplicon:

  • Sequence:

  • Size:

    108

Documents

Citation Type Local copy
Gao, H., Gadlage, M.J., Lafitte, H.R., Lenderts, B., Yang, M., Schroder, M., Farrell, J., Snopek, K., Peterson, D., Feigenbutz, L., Jones, S., St Clair, G., Rahe, M., Sanyour-Doyel, N., Peng, C., Wang, L., Young, J.K., Beatty, M., Dahlke, B., Hazebroek, J., Greene, T.W., Cigan, A.M., Chilcoat, N.D., Meeley, R.B., (2020) Superior field performance of waxy corn engineered using CRISPR–Cas9. Nature 38, 579-581.Link to the document url. peer-reviewed article