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Detection Image Detection method details

Name:

 QL-EVE-Kef6 forward/Kef6 reverse

Description:

 Qualitative real-time PCR (TaqMan) method for detection of rice event Kefeng6 (Guertler et al., 2012)

Comment:

 Target is the 3' junction between the insert of rice event Kefeng6 and the rice host genome

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 event-specific

Target GMO name:

 Kefeng6

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Kef6 forward

  • Sequence:

    TGGATCAGATTGTCGTTTCCCGCCTT

  • Size:

    26

  • Reverse Primer

  • Name:

    Kef6 reverse

  • Sequence:

    CAAGAAGCAAGCTGAGGCAAACAAGCT

  • Size:

    27

  • Probe

  • Name:

    Kef6 hydrolysis probe

  • Sequence:

    AGGGCGATTGCTGGCGAGGC

  • Size:

    20

  • Amplicon:

  • Sequence:

    tggatcagattgtcgtttcccgccttcagtttaaactatcagcgacaaaagatcaggatttgggaagggcgattgctggcgaggcacatatagctccatatagcttgtttgcctcagcttgcttcttg

  • Size:

    128

Documents

Citation Type Local copy
Guertler P., Huber I. et al. (2012) Development of an event-specific detection method for genetically modified rice Kefeng 6 by quantitative real-time PCR. J. Verbr. Lebensm. 7:63-70Link to the document url. peer-reviewed article Download PDF pdf