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Detection Image Detection method details

Name:

 QL-ELE-cry2Ab2 - F/cry2Ab2 - R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the cry2Ab2 gene (Dinon et al. 2011).

Comment:

 Target is the cry2Ab2 gene from bacterium Bacillus thuringiensis var. kumamotoensis.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
CS-cry2Ab2-BACTK

  • Oligonucleotides:

  • Forward Primer

  • Name:

    cry2Ab2 - F

  • Sequence:

    AATTCTAACTACTTCCCCGACTACTTC

  • Size:

    27

  • Reverse Primer

  • Name:

    cry2Ab2 - R

  • Sequence:

    ACGGAGAGGCGATGTTCCTG

  • Size:

    20

  • Probe

  • Name:

    cry2Ab2 - P

  • Sequence:

    TCTCTGGTGTTCCTCTCGTCGTCCGCA

  • Size:

    27

  • Amplicon:

  • Sequence:

    aattctaactacttccccgactacttcatcaggaacatctctggtgttcctctcgtcgtccgcaacgaggacctccgccgtccactgcactacaacgagatcaggaacatcgcctctccgt

  • Size:

    121

Documents

Citation Type Local copy
Dinon, A. Z., T. W. Prins, et al. (2011). Development and validation of real-time PCR screening methods for detection of cry1A.105 and cry2Ab2 genes in genetically modified organisms." Analytical and Bioanalytical Chemistry 400(5): 1433-1442.Link to the document url. peer-reviewed article