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Detection Image Detection method details

Name:

 QT-EVE-ZM-026

Description:

 Quantitative PCR method for detection of maize event 4114 (EURL GMFF GMOMETHODS database, as of May 24, 2018).

Comment:

 Target is the 5' integration border region (IBR) between the insert of maize event 4114 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-005

Type:

 event-specific

Target GMO name:

 4114

  • Oligonucleotides:

  • Forward Primer

  • Name:

    PHN164689

  • Sequence:

    GCTTTGGAGCCTCTCGTTTGTA

  • Size:

    22

  • Reverse Primer

  • Name:

    PHN164690

  • Sequence:

    GCGTTTAAACTATCAGATCTGTGTTGAA

  • Size:

    28

  • Probe

  • Name:

    PHN164691

  • Sequence:

    CACTTGCACGTAGTTACCCGGACCGAA

  • Size:

    27

  • Amplicon:

  • Sequence:

    gctttggagcctctcgtttgtancacttgcacgtagttacccggaccgaannttcaacacagatctgatagtttaaacgc

  • Size:

    80

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-026Link to the document url. EU reference method protocol