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Detection Image Detection method details

Name:

 QT-EVE-ZM-016

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event MON88017 (EU-RL GMFF GMOMETHODS database as of March 13, 2014)

Comment:

 Target is the 3' integration border region (IBR) between the insert of maize event MON88017 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-002

Type:

 event-specific

Target GMO name:

 MON88017

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MON88017 AF

  • Sequence:

    GAGCAGGACCTGCAGAAGCT

  • Size:

    20

  • Reverse Primer

  • Name:

    MON88017 AR

  • Sequence:

    TCCGGAGTTGACCATCCA

  • Size:

    18

  • Probe

  • Name:

    MON88017 AP

  • Sequence:

    TCCCGCCTTCAGTTTAAACAGAGTCGGGT

  • Size:

    29

  • Amplicon:

  • Sequence:

    gagcaggacctgcagaagctnnnnnnnnnnnnnnnnnnnnnnnnnntcccgccttcagtttaaacagagtcgggtntggatggtcaactccgga

  • Size:

    94

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-016Link to the document url. EU reference method protocol