Help icon

Detection Image Detection method details

Name:

 QT-EVE-ZM-002

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event 5307 (EURL GMFF GMOMETHODS database as of 2014).

Comment:

 Target is the 3' integration border region (IBR) between the insert of maize event 5307 and the maize host genome.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 event-specific

Target GMO name:

 5307

  • Oligonucleotides:

  • Forward Primer

  • Name:

    5307i3' forward primer

  • Sequence:

    CATGGCCGTATCCGCAATGTG

  • Size:

    21

  • Reverse Primer

  • Name:

    5307i3' reverse primer

  • Sequence:

    TGCACCCTTTGCCAGTGG

  • Size:

    18

  • Probe

  • Name:

    5307i3'-s2 probe

  • Sequence:

    ACCACAATATACCCTCTTCCCTGGGCCAG

  • Size:

    29

  • Amplicon:

  • Sequence:

    catggccgtatccgcaatgtgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaccacaatataccctcttccctgggccagnnnnnnnccactggcaaagggtgca

  • Size:

    107

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-002Link to the document url. EU reference method protocol