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Detection Image Detection method details

Name:

 QT-EVE-ZM-022

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event MIR162 (EU-RL GMFF GMOMETHODS database as of January 10, 2014)

Comment:

 Target is the 3' integration border region (IBR) between the insert of maize event MIR162 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-014

Type:

 event-specific

Target GMO name:

 MIR162

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MIR162-f1

  • Sequence:

    GCGCGGTGTCATCTATGTTACTAG

  • Size:

    24

  • Reverse Primer

  • Name:

    MIR162-r1

  • Sequence:

    TGCCTTATCTGTTGCCTTCAGA

  • Size:

    22

  • Probe

  • Name:

    MIR162-p1

  • Sequence:

    TCTAGACAATTCAGTACATTAAAAACGTCCGCCA

  • Size:

    34

  • Amplicon:

  • Sequence:

    gcgcggtgtcatctatgttactagnnnnnnnnntctagacaattcagtacattaaaaacgtccgccannntctgaaggcaacagataaggca

  • Size:

    92

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-022Link to the document url. EU reference method protocol