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Detection Image Detection method details

Name:

 QT-EVE-OS-001

Description:

 Quantitative real-time PCR (TaqMan) method for detection of rice event Golden Rice 2 (EU-RL GMFF GMOMETHODS database as of April 19, 2016)

Comment:

 Target is the 3' integration border region (IBR) between the insert of rice event Golden Rice 2 and the rice host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 event-specific

Target GMO name:

 Golden Rice 2

  • Oligonucleotides:

  • Forward Primer

  • Name:

    GR2 For

  • Sequence:

    TGGCCGTATCCGCAATGTG

  • Size:

    19

  • Reverse Primer

  • Name:

    RB-REV-BS

  • Sequence:

    GCTGCGCCTCTAACCAGGTAT

  • Size:

    21

  • Probe

  • Name:

    GR2 Probe

  • Sequence:

    TCGATATGATTCCTTCATCGAGCGAGC

  • Size:

    27

  • Amplicon:

  • Sequence:

    tggccgtatccgcaatgtgnnnnnnnnnnnnntcgatatgattccttcat cgagcgagcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn atacctggttagaggcgcagc

  • Size:

    121

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-OS-001Link to the document url. EU reference method protocol