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Detection Image Detection method details

Name:

 QT-EVE-BN-001

Description:

 Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event T45 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of oilseed rape event T45 and the oilseed rape host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-BN-012

Type:

 event-specific

Target GMO name:

 T45

  • Oligonucleotides:

  • Forward Primer

  • Name:

    KVM172

  • Sequence:

    CAATGGACACATGAATTATGC

  • Size:

    21

  • Reverse Primer

  • Name:

    MDB599

  • Sequence:

    GACTCTGTATGAACTGTTCGC

  • Size:

    21

  • Probe

  • Name:

    TM026 probe

  • Sequence:

    TAGAGGACCTAACAGAACTCGCCGT

  • Size:

    25

  • Amplicon:

  • Sequence:

    caatggacacatgaattatgcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntagaggacctaacagaactcgccgtnnnnnnnngcgaacagttcatacagagtc

  • Size:

    123

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-001Link to the document url. EU reference method protocol