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Detection Image Detection method details

Name:

 QL-EVE-Oxy-3F/Oxy-4R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of of oilseed rape event OXY-235 (Yang et al. 2008)

Comment:

 Target is the 3' integration border region (IBR) between the insert of oilseed rape event OXY-235 and the oilseed rape host genome

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

 QT-TAX-BN-012

Type:

 event-specific

Target GMO name:

 OXY-235

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Oxy-3F

  • Sequence:

    CTAACTTTTGGTGTGATGATGCTGA

  • Size:

    25

  • Reverse Primer

  • Name:

    Oxy-4R

  • Sequence:

    CGATAGATGGTGGTGTGAGTCTTG

  • Size:

    24

  • Probe

  • Name:

    Oxy-p

  • Sequence:

    AGCTGATGGCAAGTTAATCTCCCCGAAGTCG

  • Size:

    31

  • Amplicon:

  • Sequence:

    ctaacttttggtgtgatgatgctgactggcaagttaatctagtttccggttatgaagcacggcgtgtcagctgatggcaagttaatctccccgaagtcgacaagactcacaccaccatctatcg

  • Size:

    124

Documents

Citation Type Local copy
Yang L, Guo J, Zhang H, Liu J, Zhang D (2008) Qualitative and Quantitative Event-Specific PCR Detection Methods for Oxy-235 Canola Based on the 3′ Integration Flanking Sequence. J. Agric. Food Chem, 56, 1804-1809Link to the document url. peer-reviewed article