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Detection Image Detection method details

Name:

 QT-EVE-ZM-015

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of maize event Bt11 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-014

Type:

 event-specific

Target GMO name:

 Bt11

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Bt11-ev-f1

  • Sequence:

    TGTGTGGCCATTTATCATCGA

  • Size:

    21

  • Reverse Primer

  • Name:

    Bt11-ev-r5

  • Sequence:

    CGCTCAGTGGAACGAAAACTC

  • Size:

    21

  • Probe

  • Name:

    Bt11-ev-p1

  • Sequence:

    TTCCATGACCAAAATCCCTTAACGTGAGT

  • Size:

    29

  • Amplicon:

  • Sequence:

    tgtgtggccatttatcatcganttccatgaccaaaatcccttaacgtgagttttcgttccactgagcg

  • Size:

    68

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-015Link to the document url. EU reference method protocol