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Detection Image Detection method details

Name:

 QT-EVE-GM-010

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87701 (EU-RL GMFF GMOMETHODS database as of January 23, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of soybean event MON87701 and the soybean host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GM-002

Type:

 event-specific

Target GMO name:

 MON87701

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MON 87701 primer 2

  • Sequence:

    TGGTGATATGAAGATACATGCTTAGCAT

  • Size:

    28

  • Reverse Primer

  • Name:

    MON 87701 primer 1

  • Sequence:

    CGTTTCCCGCCTTCAGTTTAAA

  • Size:

    22

  • Probe

  • Name:

    MON 87701 probe

  • Sequence:

    TCAGTGTTTGACACACACACTAAGCGTGCC

  • Size:

    30

  • Amplicon:

  • Sequence:

    tggtgatatgaagatacatgcttagcatnnnnnnggcacgcttagtgtgtgtgtcaaacactgannntttaaactgaaggcgggaaacg

  • Size:

    89

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-010Link to the document url. EU reference method protocol