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Detection Image Detection method details

Name:

 QT-EVE-GM-009

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean event DP356043 (EU-RL GMFF GMOMETHODS database as of January 23, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of soybean event DP356043 and the soybean host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GM-002

Type:

 event-specific

Target GMO name:

 DP356043

  • Oligonucleotides:

  • Forward Primer

  • Name:

    DP356-f1

  • Sequence:

    GTCGAATAGGCTAGGTTTACGAAAAA

  • Size:

    26

  • Reverse Primer

  • Name:

    DP356-r1

  • Sequence:

    TTTGATATTCTTGGAGTAGACGAGAGTGT

  • Size:

    29

  • Probe

  • Name:

    DP356-p

  • Sequence:

    CTCTAGAGATCCGTCAACATGGTGGAGCAC

  • Size:

    30

  • Amplicon:

  • Sequence:

    gtcgaataggctaggtttacgaaaaannnnnnnnnnnnnctctagagatccgtcaacatggtggagcacnacactctcgtctactccaagaatatcaaa

  • Size:

    99

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-009Link to the document url. EU reference method protocol