Help icon

Detection Image Detection method details

Name:

 QL-TAX-OS-002

Description:

 Qualitative real-time PCR (TaqMan) method for detection of rice Phospholipase D alpha 2 gene (Mazzara et al., 2006).

Comment:

 target is the endogenous phospholipase D (PLD) gene of rice

Validation:

 in-house validation

Standardisation:

 unknown

Related Methods:

Type:

 taxon-specific

Target Species name:

 Oryza sativa

  • Oligonucleotides:

  • Forward Primer

  • Name:

    KVM159

  • Sequence:

    TGGTGAGCGTTTTGCAGTCT

  • Size:

    20

  • Reverse Primer

  • Name:

    KVM160

  • Sequence:

    CTGATCCACTAGCAGGAGGTCC

  • Size:

    22

  • Probe

  • Name:

    TM013

  • Sequence:

    TGTTGTGCTGCCAATGTGGCCTG

  • Size:

    23

  • Amplicon:

  • Sequence:

    tggtgagcgttttgcagtctntgttgtgctgccaatgtggcctgnnggacctcctgctagtggatcag

  • Size:

    68

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-TAX-OS-002Link to the document url. EU reference method protocol