Detection method details
Name: |
QT-EVE-BN-014 |
Description: |
Quantitative real-time PCR (TaqMan) for detection of oilseed rape event MON94100 (EU-RL GMFF GMOMETHODS database as of October 12, 2022) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of oilseed rape event MON94100 and the oilseed rape host genome. |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-BN-001 |
Type: |
event-specific |
Target GMO name: |
MON94100 |
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Oligonucleotides:
Forward Primer
Name:
MON 94100 primer 1Sequence:
CACCATCTAATGAATAGTCACCAAAATAACGSize:
31
Reverse Primer
Name:
MON 94100 primer 2Sequence:
CTATTCGGGCCTAACTTTTGGTGTGSize:
25
Probe
Name:
MON 94100 probeSequence:
TGATGCTGACTGGTGTCAASize:
19
-
Amplicon:
Sequence:
caccatctaa tgaatagtca ccaaaataac gnnnnnnnnn nnnnttgaca ccagtcagca tcancacacc aaaagttagg cccgaatagSize:
89
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-014 | EU reference method protocol |