Detection method details
Name: |
QL-EVE-CP-001 |
Description: |
Qualitative conventional PCR method for the detection of papaya event Huanong No. 1 (EU-RL GMFF GMOMETHODS database as of September 19, 2016) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of papaya event Huanong No. 1 and the papaya host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
event-specific |
Target GMO name: |
Huanong No. 1 |
-
Oligonucleotides:
Forward Primer
Name:
HN-FSequence:
ATTGACGAGTACAAGGAGACGCSize:
22
Reverse Primer
Name:
HN-RSequence:
CTCCGCTCATGATCAGATTGTSize:
21
Probe
Name:
Sequence:
Size:
-
Amplicon:
Sequence:
attgacgagtacaaggagacgccttttaatttgtttaaattaaataaaaacataaattattaagcgacgtaataataaacacgtcataaattttttcaaaaaataaaatttcttttattttctaaaaagtagttgattcgcccaatagcagccagtcccttcccgcttcagtgacaacgtcgagcacagctgcgcaaggaacgcccgtcgtggccagccacgatagcctcaaacactgatagtttaaactgaaggcgggaaacgacaatctgatcatgagcggagSize:
285
Documents
Citation | Type | Local copy |
---|---|---|
Wei, J. et al. (2016). Collaborative trial for the validation of event-specific PCR detection methods of genetically modified papaya Huanong No. 1. Food Chemistry, 194, 20-25. | peer-reviewed article | |
EU-RL GMFF GMOMETHODS database; QL-EVE-CP-001 | EU reference method protocol |