Detection method details
Name: |
QL-TAX-CMV-001 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of CaMV ORF V (ISO/TS 21569-7, 2022). |
Comment: |
Target is the open reading frame V (ORF V) from Cauliflower mosaic virus (CaMV) |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Cauliflower mosaic virus |
-
Oligonucleotides:
Forward Primer
Name:
CaMV-ORFV-fd2Sequence:
ATYAAGCCCAGYAAAAGCCCSize:
20
Reverse Primer
Name:
CaMV-ORFV-rd2Sequence:
CTYCGCTTCTCGGCTTCRTTSize:
20
Probe
Name:
CaMV-ORFV-P2Sequence:
CATGGCACCAGCCTTCTTGGTCAACSize:
25
-
Amplicon:
Sequence:
atyaagcccagyaaaagcccnnncatggcaccagccttcttggtcaacaaygaagccgagaagcgragSize:
68
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QL-TAX-CMV-001 | EU reference method protocol |