Detection method details
Name: |
QL-ELE-Cry1F-F2/Cry1Fr-n2 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the cry1F gene (Scholtens et al., 2013, with minor modifications in forward primer and probe sequence) |
Comment: |
Target is the cry1F gene from bacterium Bacillus thuringiensis var. aizawai. |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-cry1F_tr-BACTA
|
-
Oligonucleotides:
Forward Primer
Name:
Cry1F-F2Sequence:
GACGTGGATCTTCATCTGCAATCSize:
23
Reverse Primer
Name:
Cry1Fr-n2Sequence:
GCAACACGGCTGGCAATCGSize:
19
Probe
Name:
Cry1F-P2Sequence:
CGCCCCCGGGATTGAAGACCCCGTAACSize:
27
-
Amplicon:
Sequence:
GACGTGGATCTTCATCTGCAATCCAGATGGCGCCCCCGGGATTGAAGACCCCGTAACTAGGAAAGTTGATACGATTGCCAGCCGTGTTGCSize:
90
Documents
Citation | Type | Local copy |
---|---|---|
Scholtens et al. (2012) |