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Detection Image Detection method details

Name:

 QT-EVE-ZM-039

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event DP51291 (EU-RL GMFF GMOMETHODS database as of June 13, 2024).

Comment:

 Target is the 3' integration border region (IBR) between the insert of maize event DP51291 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-008

Type:

 event-specific

Target GMO name:

 DP51291

  • Oligonucleotides:

  • Forward Primer

  • Name:

    DP-051291-2 Forward Primer

  • Sequence:

    CGCAATGTGTTATTAAGTTGTCTAAGC

  • Size:

    27

  • Reverse Primer

  • Name:

    DP-051291-2 Reverse Primer

  • Sequence:

    CACGGTTAGCTGTACCAAATGC

  • Size:

    22

  • Probe

  • Name:

    DP-051291-2 Probe

  • Sequence:

    TTTGTTTACACCACATACACG

  • Size:

    21

  • Amplicon:

  • Sequence:

    cgcaatgtgttattaagttgtctaagcnnnnntttgtttacaccacatacacgnnnnngcatttggtacagctaaccgtg

  • Size:

    80

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-039Link to the document url. PCR Protocol