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Detection Image Detection method details

Name:

 QT-EVE-ZM-037

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event MON94804 (EU-RL GMFF GMOMETHODS database as of June 7, 2024).

Comment:

 Target is the 5' integration border region (IBR) between the insert of maize event MON94804 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-002

Type:

 event-specific

Target GMO name:

 MON94804

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MON94804 primer 1

  • Sequence:

    CTCTTCTAATCCGGGCCATCG

  • Size:

    21

  • Reverse Primer

  • Name:

    MON94804 primer 2

  • Sequence:

    AGTTAGTCGCGCCAAATCGTG

  • Size:

    21

  • Probe

  • Name:

    MON94804 probe

  • Sequence:

    CTGGATCCGAAGGACGTGTCTACATTCAC

  • Size:

    29

  • Amplicon:

  • Sequence:

    ctcttctaatccgggccatcgnnnctggatccgaaggacgtgtctacattcacnnnnnnnnnnnnnnnnnnnnnnnnnnnnncacgatttggcgcgactaact

  • Size:

    103

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-037Link to the document url. EU reference method protocol