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Detection Image Detection method details

Name:

 QL-ELE-P-Ubi-F/P-Ubi-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of ubiquitin promoter of Zea mays (Debode et al., 2013)

Comment:

 Target is the ubiquitin promoter (P-ubi) from Zea mays

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
P-ubi1-MAIZE

  • Oligonucleotides:

  • Forward Primer

  • Name:

    P-Ubi-F

  • Sequence:

    GAGTAGATAATGCCAGCCTGTTAAAC

  • Size:

    26

  • Reverse Primer

  • Name:

    P-Ubi-R

  • Sequence:

    ACGCGACGCTGCTGGTT

  • Size:

    17

  • Probe

  • Name:

    P-Ubi-P

  • Sequence:

    CGTCGACGAGTCTAACGGACACCAAC

  • Size:

    26

  • Amplicon:

  • Sequence:

    GAGTAGATAATGCCAGCCTGTTAAACGCCGTCGACGAGTCTAACGGACACCAACCAGCGAACCAGCAGCGTCGCGT

  • Size:

    76

Documents

Citation Type Local copy