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Detection Image Detection method details

Name:

 QL-EVE-DC-003

Description:

 Qualitative conventional PCR method for the detection of carnation event 25958 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2015/692) (EURL GMFF GMOMETHODS database as of June 1, 2016)

Comment:

 Target is the 3' integration border region (IBR) between the insert of carnation event 25958 and the carnation host genome.

Validation:

 in-house validation

Standardisation:

 EU reference method

Related Methods:

 QL-TAX-DC-001

Type:

 event-specific

Target GMO name:

 25958

  • Oligonucleotides:

  • Forward Primer

  • Name:

    RB Forward

  • Sequence:

    TGGACCCTTGAGGAAACTGGTAGC

  • Size:

    24

  • Reverse Primer

  • Name:

    IFD-25958-3 Reverse

  • Sequence:

    CTTGTACACCGCGTTATGCTC

  • Size:

    21

  • Amplicon:

  • Sequence:

    tggacccttgaggaaactggtagcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngagcataacgcggtgtacaag

  • Size:

    482

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-EVE-DC-003Link to the document url. EU reference method protocol