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Detection Image Detection method details

Name:

 QL-ELE-00-011

Description:

 Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).

Comment:

 Target is the nopaline synthase terminator (T-nos) from Agrobacterium tumefaciens.

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 element-specific

Target DNA element:
T-nos-RHIRD

  • Oligonucleotides:

  • Forward Primer

  • Name:

    180-F

  • Sequence:

    CATGTAATGCATGACGTTATTTATG

  • Size:

    25

  • Reverse Primer

  • Name:

    180-R

  • Sequence:

    TTGTTTTCTATCGCGTATTAAATGT

  • Size:

    25

  • Probe

  • Name:

    Tm-180

  • Sequence:

    ATGGGTTTTTATGATTAGAGTCCCGCAA

  • Size:

    28

  • Amplicon:

  • Sequence:

    catgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaa

  • Size:

    84

Documents

Citation Type Local copy
Reiting R., Broll H., Waiblinger H.-U., Grohmann L.; Collaborative Study of a T-nos Real-Time PCR Method for Screening of Genetically Modified Organisms in Food Products J. Verbr. Lebensm. 2:116-121 (2007)Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QL-ELE-00-011Link to the document url. EU reference method protocol