Detection method details
Name: |
QT-EVE-ZM-012 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event DAS59122 (EU-RL GMFF GMOMETHODS database as of March 13, 2014) |
Comment: |
Target is the integration border region (IBR) between the insert of maize event DAS59122 and the maize host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-ZM-002 |
Type: |
event-specific |
Target GMO name: |
DAS59122 |
-
Oligonucleotides:
Forward Primer
Name:
DAS-59122-7-rb1fSequence:
GGGATAAGCAAGTAAAAGCGCTCSize:
23
Reverse Primer
Name:
DAS-59122-rb1rSequence:
CCTTAATTCTCCGCTCATGATCAGSize:
24
Probe
Name:
DAS-59122-7-rb1sSequence:
TTTAAACTGAAGGCGGGAAACGACAASize:
26
-
Amplicon:
Sequence:
gggataagcaagtaaaagcgctcnnnnnnnnnnnntttaaactgaaggcgggaaacgacaanctgatcatgagcggagaattaaggSize:
86
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-012 | EU reference method protocol |