Detection method details
Name: |
QL-ELE-cry2Ab2 - F/cry2Ab2 - R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the cry2Ab2 gene (Dinon et al. 2011). |
Comment: |
Target is the cry2Ab2 gene from bacterium Bacillus thuringiensis var. kumamotoensis. |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-cry2Ab2-BACTK
|
-
Oligonucleotides:
Forward Primer
Name:
cry2Ab2 - FSequence:
AATTCTAACTACTTCCCCGACTACTTCSize:
27
Reverse Primer
Name:
cry2Ab2 - RSequence:
ACGGAGAGGCGATGTTCCTGSize:
20
Probe
Name:
cry2Ab2 - PSequence:
TCTCTGGTGTTCCTCTCGTCGTCCGCASize:
27
-
Amplicon:
Sequence:
aattctaactacttccccgactacttcatcaggaacatctctggtgttcctctcgtcgtccgcaacgaggacctccgccgtccactgcactacaacgagatcaggaacatcgcctctccgtSize:
121