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Detection Image Detection method details

Name:

 QT-EVE-ZM-011

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event T25 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)

Comment:

 Target is the 3' integration border region (IBR) between the insert of maize event T25 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-003

Type:

 event-specific

Target GMO name:

 T25

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MLD143

  • Sequence:

    ACAAGCGTGTCGTGCTCCAC

  • Size:

    20

  • Reverse Primer

  • Name:

    MDB551

  • Sequence:

    GACATGATACTCCTTCCACCG

  • Size:

    21

  • Probe

  • Name:

    TM016

  • Sequence:

    TCATTGAGTCGTTCCGCCATTGTCG

  • Size:

    25

  • Amplicon:

  • Sequence:

    acaagcgtgtcgtgctccacnnnnnnnnnnnnnnnnnnnnnnnnntcattgagtcgttccgccattgtcgnnnnnnnnnnncggtggaaggagtatcatgtc

  • Size:

    102

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-011Link to the document url. EU reference method protocol