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Detection Image Detection method details

Name:

 QT-EVE-GM-012

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87708 (EU-RL GMFF GMOMETHODS database as of January 27, 2014)

Comment:

 Target is the 3' integration border region (IBR) between the insert of soybean event MON87708 and the soybean host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GM-002

Type:

 event-specific

Target GMO name:

 MON87708

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MON87708 primer 1

  • Sequence:

    TCATACTCATTGCTGATCCATGTAG

  • Size:

    25

  • Reverse Primer

  • Name:

    MON87708 primer 2

  • Sequence:

    AGAACAAATTAACGAAAAGACAGAACG

  • Size:

    27

  • Probe

  • Name:

    MON87708 prob

  • Sequence:

    TCCCGGACTTTAGCTCAAAATGCATGTA

  • Size:

    28

  • Amplicon:

  • Sequence:

    tcatactcattgctgatccatgtagnnntcccggactttagctcaaaatgcatgtannnnnnnncgttctgtcttttcgttaatttgttct

  • Size:

    91

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-012Link to the document url. EU reference method protocol