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Detection Image Detection method details

Name:

 QL-EVE-18-2-4RB-F/18-2-4RB-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of papaya event 18-2-4 (Prins et al., 2016)

Comment:

 Target is the 3' integration border region (IBR) between the insert of papaya event 18-2-4 and the papaya host genome.

The nucleotide sequences of the 309 bp spanning the Right Border of event 18-2-4 is given in GenBank KU376439.

Validation:

 unknown

Standardisation:

 none

Related Methods:

Type:

 event-specific

Target GMO name:

 18-2-4

  • Oligonucleotides:

  • Forward Primer

  • Name:

    18-2-4RB-F

  • Sequence:

    AGGAGACGACGACAA

  • Size:

    15

  • Reverse Primer

  • Name:

    18-2-4RB-R

  • Sequence:

    TCTCCGCTCATGATCA

  • Size:

    16

  • Probe

  • Name:

    18-2-4RB-P

  • Sequence:

    ATTGTCGTTTCCCGCCTTCAG

  • Size:

    21

  • Amplicon:

  • Sequence:

    AGGAGACGACGACAAAAATAGTAGTAGTGATGAAGAAAATAACAAAATAACAATTATGAGAACAAAGTGATGATAACATCAATAACAAATTAATAAATAATAAGATAGTTTAAACTGAAGGCGGGAAACGACAATCTGATCATGAGCGGAGA

  • Size:

    152

Documents

Citation Type Local copy
Prins, T.W., Scholtens, I.M.J, Bak, A.W., van Dijk, J.P., Voorhuijzen, M.M., Laurensse, E.J., Kok, E.J. (2016). A case study to determine the geographical origin of unknown GM papaya in routine food sample analysis, followed by identification of papaya events 16-0-1 and 18-2-4. Food Chemistry, 213, 536–544.Link to the document url. peer-reviewed article