Help icon

Detection Image Detection method details

Name:

 QT-ELE-00-003

Description:

 Quantitative real-time PCR (TaqMan) method for detection of synthetic cryIA(b) gene (ISO/FDIS 21570)

Comment:

 Target is the synthetic cryIA(b) gene

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 element-specific

Target DNA element:
CS-cry1Ab-BACTU

  • Oligonucleotides:

  • Forward Primer

  • Name:

    CRY2-F

  • Sequence:

    CCCATCGACATCAGCCTGAGC

  • Size:

    21

  • Reverse Primer

  • Name:

    CRY2-R

  • Sequence:

    CAGGAAGGCGTCCCACTGGC

  • Size:

    20

  • Probe

  • Name:

    BTSYN

  • Sequence:

    ATGTCCACCAGGCCCAGCACG

  • Size:

    21

  • Amplicon:

  • Sequence:

    cccatcgacatcagcctgagcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngccagtgggacgccttcctg

  • Size:

    129

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-ELE-00-003Link to the document url. EU reference method protocol