Detection method details
Name: |
QL-TAX-OS-002 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of rice Phospholipase D alpha 2 gene (Mazzara et al., 2006). |
Comment: |
target is the endogenous phospholipase D (PLD) gene of rice |
Validation: |
in-house validation |
Standardisation: |
unknown |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Oryza sativa |
-
Oligonucleotides:
Forward Primer
Name:
KVM159Sequence:
TGGTGAGCGTTTTGCAGTCTSize:
20
Reverse Primer
Name:
KVM160Sequence:
CTGATCCACTAGCAGGAGGTCCSize:
22
Probe
Name:
TM013Sequence:
TGTTGTGCTGCCAATGTGGCCTGSize:
23
-
Amplicon:
Sequence:
tggtgagcgttttgcagtctntgttgtgctgccaatgtggcctgnnggacctcctgctagtggatcagSize:
68
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QL-TAX-OS-002 | EU reference method protocol |