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Detection Image Detection method details

Name:

 QL-ELE-cry1A 4-5'/cry1A 4-3'

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the cry1Ab gene (Scholtens et al. 2013).

Comment:

 Target is the cry1Ab gene from bacterium Bacillus thuringiensis subsp. kurstaki (Btk).

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
CS-cry1Ab-BACTU

  • Oligonucleotides:

  • Forward Primer

  • Name:

    cry1A 4-5'

  • Sequence:

    GGACAACAACCCMAACATCAAC

  • Size:

    22

  • Reverse Primer

  • Name:

    cry1A 4-3'

  • Sequence:

    GCACGAACTCGCTSAGCAG

  • Size:

    19

  • Probe

  • Name:

    Cry1A(b)-probe

  • Sequence:

    CATCCCGTACAACTGCCTCAGCAACCCTG

  • Size:

    29

  • Amplicon:

  • Sequence:

    ggacaacaacccaaacatcaacgagtgcatcccgtacaactgcctcagcaaccctgaggtcgaggtgctcggcggtgagcgcatcgagaccggttacacccccatcgacatctccctctccctcacgcagttcctgctcagcgagttcgtgc

  • Size:

    152

Documents

Citation Type Local copy