Detection method details
Name: |
QL-ELE-00-014 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (ISO 21569 AMD1) |
Comment: |
Target is the phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from the bacterium Streptomyces hygroscopicus |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-bar-STRHY
|
-
Oligonucleotides:
Forward Primer
Name:
RapB-F1Sequence:
acaagcacggtcaacttccSize:
19
Reverse Primer
Name:
RapB-R1Sequence:
gaggtcgtccgtccactcSize:
18
Probe
Name:
RapB-S1Sequence:
taccgagccgcaggaaccSize:
18
-
Amplicon:
Sequence:
acaagcacggtcaacttccgtaccgagccgcaggaaccgcaggagtggacggacgacctcSize:
60
Documents
Citation | Type | Local copy |
---|---|---|
Grohmann L., Brunen-Nieweler C., Nemeth A., Waiblinger H.U.; Collaborative trial validation studies of real-time PCR-based GMO screening methods for detection of the bar gene and the ctp2-cp4epsps construct J. Agric. Food Chem. 57:8913-8920 (2009) | peer-reviewed article | |
EU-RL GMFF GMOMETHODS database; QL-ELE-00-014 | EU reference method protocol |