Detection method details
Name: |
QL-EVE-18-2-4RB-F/18-2-4RB-R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of papaya event 18-2-4 (Prins et al., 2016) |
Comment: |
Target is the 3' integration border region (IBR) between the insert of papaya event 18-2-4 and the papaya host genome.
The nucleotide sequences of the 309 bp spanning the Right Border of event 18-2-4 is given in GenBank KU376439. |
Validation: |
unknown |
Standardisation: |
none |
Related Methods: |
Type: |
event-specific |
Target GMO name: |
18-2-4 |
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Oligonucleotides:
Forward Primer
Name:
18-2-4RB-FSequence:
AGGAGACGACGACAASize:
15
Reverse Primer
Name:
18-2-4RB-RSequence:
TCTCCGCTCATGATCASize:
16
Probe
Name:
18-2-4RB-PSequence:
ATTGTCGTTTCCCGCCTTCAGSize:
21
-
Amplicon:
Sequence:
AGGAGACGACGACAAAAATAGTAGTAGTGATGAAGAAAATAACAAAATAACAATTATGAGAACAAAGTGATGATAACATCAATAACAAATTAATAAATAATAAGATAGTTTAAACTGAAGGCGGGAAACGACAATCTGATCATGAGCGGAGASize:
152