Detection method details
Name: |
QL-CON-cptiF2/tNOS-uni-R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the cpti gene and nos terminator (Reiting et al., 2010) |
Comment: |
Target is the junction between between the cow pea trypsin inhibitor (cpti) gene and the nopaline synthase gene terminator (T-nos)from Agrobacterium tumefaciens |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
construct-specific |
Target GMO names: |
Kefeng6, SGK321
|
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Oligonucleotides:
Forward Primer
Name:
cptiF2Sequence:
caagtccagggatgaagatgatgagSize:
25
Reverse Primer
Name:
tNOS-uni-RSequence:
gcttaacgtaattcaacagaaattatatgataatSize:
34
Probe
Name:
tNOS-uni-TmSequence:
tcgcaagaccggcaacaggattcSize:
23
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Amplicon:
unknown