Detection method details
Name: |
QT-TAX-SPS primer f/SPS primer r |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of the rice sucrose phosphate synthase (SPS) gene (ISO21570 Amd1) |
Comment: |
Target is the endogenous sucrose phosphate synthase (SPS) gene from Oryza sativa |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Oryza sativa |
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Oligonucleotides:
Forward Primer
Name:
SPS primer fSequence:
TTGCGCCTGAACGGATATSize:
18
Reverse Primer
Name:
SPS primer rSequence:
CGGTTGATCTTTTCGGGATGSize:
20
Probe
Name:
SPS probeSequence:
TCCGAGCCGTCCGTGCGTCSize:
19
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Amplicon:
Sequence:
ttgcgcctgaacggatatctttcagtttgtaaccaccggatgacgcacggacggctcggatcatcccgaaaagatcaaccgSize:
81