Help icon

Detection Image Detection method details

Name:

 QT-TAX-ZM-006

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (ISO 21570)

Comment:

 Target is the endogenous starch synthase IIb (zSSIIb) gene from Zea mays

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 taxon-specific

Target Species name:

 Zea mays

  • Oligonucleotides:

  • Forward Primer

  • Name:

    SSllb 1-5'

  • Sequence:

    CTCCCAATCCTTTGACATCTGC

  • Size:

    22

  • Reverse Primer

  • Name:

    SSIIb 1-3'

  • Sequence:

    TCGATTTCTCTCTTGGTGACAGG

  • Size:

    23

  • Probe

  • Name:

    SSIIb-Taq

  • Sequence:

    AGCAAAGTCAGAGCGCTGCAATGCA

  • Size:

    25

  • Amplicon:

  • Sequence:

    ctcccaatcctttgacatctgcnnnnnagcaaagtcagagcgctgcaatgcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncctgtcaccaag agagaaatcga

  • Size:

    151

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-TAX-ZM-006Link to the document url. EU reference method protocol
Shindo, Y., Kuribara, H., Matsuoka, T., Futo, S., Sawada, C., Shono, J., Akiyama, H., Goda, Y., Toyoda, M., Hino, A. (2002). Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules. Journal of the AOAC International, 85, 1119-1126Link to the document url. peer-reviewed article