Help icon

Detection Image Detection method details

Name:

 QL-TAX-SL-001

Description:

 Qualitative conventional PCR method for detection of tomato polygalacturonase gene (ISO/FDIS 21569:2005).

Comment:

 target is the endogenous polygalacturonase (PG) gene of tomato

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 taxon-specific

Target Species name:

 Solanum lycopersicum

  • Oligonucleotides:

  • Forward Primer

  • Name:

    PG34L

  • Sequence:

    GGATCCTTAGAAGCATCTAGT

  • Size:

    21

  • Reverse Primer

  • Name:

    PG34R

  • Sequence:

    CGTTGGTGCATCCCTGCATGG

  • Size:

    21

  • Amplicon:

  • Sequence:

    ggatccttagaagcatctagtnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnccatgcagggatgcaccaacg

  • Size:

    384

Documents

Citation Type Local copy
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods ISO 21569:1-69 (2005)Link to the document url. other document
EU-RL GMFF GMOMETHODS database; QL-TAX-SL-001Link to the document url. EU reference method protocol
Screening procedure for the detection of genetically modified DNA sequences in foods by identification of DNA sequences that frequently occur in genetically modified organisms. BVL Official Method Collection L 00.00-31 Online Publication (2001)Link to the document url. other document 11-05-2016