Detection method details
Name: |
QL-TAX-OS-003 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of rice root-specific GOS9 gene (Hernandez et al., 2005) |
Comment: |
target is the endogenous root-specific GOS9 gene of rice |
Validation: |
ring trial validation |
Standardisation: |
national standard |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Oryza sativa |
-
Oligonucleotides:
Forward Primer
Name:
org1Sequence:
TTAGCCTCCCGCTGCAGASize:
18
Reverse Primer
Name:
org2Sequence:
AGAGTCCACAAGTGCTCCCGSize:
20
Probe
Name:
orgpSequence:
CGGCAGTGTGGTTGGTTTCTTCGGSize:
24
-
Amplicon:
Sequence:
ttagcctcccgctgcagancggcagtgtggttggtttcttcggnnnnncgggagcacttgtggactctSize:
68
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QL-TAX-OS-003 | EU reference method protocol |