Help icon

Detection Image Detection method details

Name:

 QL-ELE-AINT 2-5'/AINT 2-3'

Description:

 Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 intron (I-ractI) (Mano et al. 2009).

Comment:

 Target is the actin 1 intron from rice (Oryza sativa).

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
I-actin

  • Oligonucleotides:

  • Forward Primer

  • Name:

    AINT 2-5'

  • Sequence:

    TCGTCAGGCTTAGATGTGCTAGA

  • Size:

    23

  • Reverse Primer

  • Name:

    AINT 2-3'

  • Sequence:

    CTGCATTTGTCACAAATCATGAA

  • Size:

    23

  • Probe

  • Name:

    AINT-2-Taq

  • Sequence:

    TTTGTGGGTAGAATTTGAATCCCTCAGC

  • Size:

    28

  • Amplicon:

  • Sequence:

    tcgtcaggcttagatgtgctagatctttctttcttctttttgtgggtagaatttgaatccctcagcattgttcatcggtagtttttcttttcatgatttgtgacaaatgcag

  • Size:

    112

Documents

Citation Type Local copy
Mano J, Shigemitsu N, Futo S, Akiyama H, Teshima R, Hino A, Furui S, Kitta K (2009) Real-Time PCR array as a universal platform for the detection of genetically modified crops and its application in identifying unapproved genetically modified crops in Japan, Journal of Agricultural and Food Chemistry, 57, 26-37Link to the document url. peer-reviewed article