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Detection Image Detection method details

Name:

 QL-CON-cptiF2/tNOS-uni-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the junction between the cpti gene and nos terminator (Reiting et al., 2010)

Comment:

 Target is the junction between between the cow pea trypsin inhibitor (cpti) gene and the nopaline synthase gene terminator (T-nos)from Agrobacterium tumefaciens

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 construct-specific

Target GMO names:
Kefeng6, SGK321

  • Oligonucleotides:

  • Forward Primer

  • Name:

    cptiF2

  • Sequence:

    caagtccagggatgaagatgatgag

  • Size:

    25

  • Reverse Primer

  • Name:

    tNOS-uni-R

  • Sequence:

    gcttaacgtaattcaacagaaattatatgataat

  • Size:

    34

  • Probe

  • Name:

    tNOS-uni-Tm

  • Sequence:

    tcgcaagaccggcaacaggattc

  • Size:

    23

  • Amplicon:

    unknown

Documents

Citation Type Local copy
Reiting R, Grohmann L, Maede D (2010) A testing cascade for the detection of genetically modified rice by real-time PCR in food and its application for detection of an unauthorized rice line similar to KeFeng6. J. Verbr. Lebensm. 5:185-188Link to the document url. peer-reviewed article