Detection method details
Name: |
QL-ELE-cry1A 4-5'/cry1A 4-3' |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the cry1Ab gene (Scholtens et al. 2013). |
Comment: |
Target is the cry1Ab gene from bacterium Bacillus thuringiensis subsp. kurstaki (Btk). |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-cry1Ab-BACTU
|
-
Oligonucleotides:
Forward Primer
Name:
cry1A 4-5'Sequence:
GGACAACAACCCMAACATCAACSize:
22
Reverse Primer
Name:
cry1A 4-3'Sequence:
GCACGAACTCGCTSAGCAGSize:
19
Probe
Name:
Cry1A(b)-probeSequence:
CATCCCGTACAACTGCCTCAGCAACCCTGSize:
29
-
Amplicon:
Sequence:
ggacaacaacccaaacatcaacgagtgcatcccgtacaactgcctcagcaaccctgaggtcgaggtgctcggcggtgagcgcatcgagaccggttacacccccatcgacatctccctctccctcacgcagttcctgctcagcgagttcgtgcSize:
152