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Detection Image Detection method details

Name:

 QL-ELE-00-020

Description:

 Qualitative real-time PCR (SYBRGreen) method for detection of CS-cry1Ab-BACTU (Barbau-Piednoir et al., 2014)

Comment:

 Target is CS-cry1Ab-BACTU from Bacillus thuringiensis

Validation:

 ring trial validation

Standardisation:

 unknown

Related Methods:

 QL-TAX-BN-003, QL-TAX-GM-001, QL-TAX-ZM-002

Type:

 element-specific

Target DNA element:
CS-cry1Ab-BACTU

  • Oligonucleotides:

  • Forward Primer

  • Name:

    CryIAb_Bt_Cott_Fwd

  • Sequence:

    ACCGGTTACACTCCCATCGA

  • Size:

    20

  • Reverse Primer

  • Name:

    CryIAb_Bt_Cott_Rev

  • Sequence:

    CAGCACCTGGCACGAACTC

  • Size:

    19

  • Amplicon:

  • Sequence:

    accggttacactcccatcgannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngagttcgtgccaggtgctg

  • Size:

    73

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-ELE-00-020Link to the document url. EU reference method protocol
Barbau-Piednoir, E., Stragier, P., Roosens, N., Mazzara, M., Savini, C., Van den Eede, G., Van den Bulcke, M. (2014). Inter-laboratory testing of GMO detection by combinatory SYBR®Green PCR screening (CoSYPS). Food Analytical Methods. 7, 1719-1728.Link to the document url. peer-reviewed article
Barbau-Piednoir, E. et al. (2012). Four new SYBR_Green qPCR screening methods for the detection of Roundup Ready, LibertyLink, and CryIAb traits in genetically modified products. Eur Food Res Technol, 234, 13–23.Link to the document url. peer-reviewed article
Grisolia, C. K., Oliveira, R., Domingues, I., Oliveira-Filho, E. C., Monerat, R. G., & Soares, A. M. V. M. (2009). Genotoxic evaluation of different delta-endotoxins from Bacillus thuringiensis on zebrafish adults and development in early life stages. Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 672(2), 119-123.Link to the document url. peer-reviewed article